Chap.26 Biotechnology MCQs

 

1.       

Biotechnology

 

2.       

Which type of ecosystem has the smallest raction of nutrients in the soil?

(a) Savanna                  (b) Tundra

(c) Desert                     (d) None of these

C

3.       

Which biome has the richest soil with nutrients?

(a) Coniferous forest   (b) Grassland

(c) Desert                     (d) None of these

A

4.       

Fresh water ecosystem covers less than:

(a) 1%                          (b) 3%

(C) 5%                         (d) None

A

5.       

It is divided into littoral, limnetic, profundal Zones

(a) Freshwater lake      (b) Desert

(c) Grassland               (d) None of these

A

6.       

Which one is not correct for littoral zone?

(a) Shallow water         (b) Plankton

(c) Detritivores             (d) None of these

A

7.       

Which one is not related with eutrophication?

(a) Depletion of                       (b) Foul Smell

(c) Fish Dominant        (d) None of these

C

8.       

Average rainfall in temperate deciduous forest is:

(a) 500-1000 mm         (b) 750-1500 mm

(c) 1500-3000 mm       (d) None of these

B

9.       

Northern charms forests are called

(a) Alpine                    (b) Taiga

©) Tundra                    (d) None of these

A

10.   

Grassland in temperate climate is called:

(a) Prairie                     (b) Savanna

(c) Taiga                      (d) None of these

A

11.   

The dominant plant of grassland is:.

(a) Shrubs                    (b) Graminnoid

(c) Legumes                 (d) None of these

B

12.   

The type of gel most commonly used for short fragment DNA electrophoresis is:

(a) Agarose                  (b) DNA Polymerase

(c) Polyacrylamide       (d) DNA Ligase

C

13.   

Cell suspension culture of Cinchona ledgeriana produces:

(a) Quinine                  (b) Digitoxin

(c) Polludrin                 (d) Antitoxin

A

14.   

Dideoxy ribonucleoside triphosphates are used to terminate DNA synthesis at different site Which method involves this procedure?

(a) Maxam - Gilbert's Method

(b) Sanger's Method

(c) K.B Mullis Method

(d) Gottlieb's Method

A

15.   

The gene of choice can also be synthesized in the laboratory from mRNA, using reverse transcriptase. This DNA molecule is called:

(a) Complementary DNA

(b) Replicative DNA

(c) Synthetic DNA

(d) SS DNA

A

16.   

The gene of interest is joined with the sticky ends produced after cutting the plasmid with

the help of another special enzyme known as:

(a) DNA Ligase

(b) DNA Polymerase

(c) Restriction Endonuclease

(d) Reverse Transcriptase

A

17.   

The enzyme DNA polymerase can work only in:

(a) 3'-5 Direction         (b) 5-3 Direction

(c) Both the Directions (d) 5 - 5 Direction

B

18.   

In recombinant DNA technology, a plasmid vector is cleaved by:

(a) Modified DNA ligase

(b) A heated alkaline solution

(c) The same enzyme that cleaves the donor DNA

(d) The different enzyme other than that cleave the donor DNA.

C

19.   

Thermus aquaticus is the source of:

(a) Taq Polymerase      (b) Vent Polymerase

(c) Botha and b                        (d) Primase Enzyme

A

20.   

The complete set of chromosomal and extra chromosomal genes of an organism is called:

(a) Genome                  (b) Gene Pool

(c) Gene Bank             (d) Gene Library

A

21.   

A totipotent cell means

(a) An undifferentiated cell capable of developing into a system or entire plant

(b) An undifferentiated cell capable of developing into an organ

(c) An undifferentiated cell capable of developing into complete embryo

(d) Cell which lacks the capability to differentiate into an organ or system

B

22.   

 Naturally restriction enzymes are found in

(a) Bacteria                  (b) Virus

(c)Fungi                       (d) All of these

A

23.   

Smal is an example of an enzyme that generates

(a) Sticky Ends                        (b) Blunt Ends

(c) Overhangs              (d) None of these

B

24.   

Plasmid PBR322 carries antibiotic resistant gene:

(a) Ampicillin              (b) Tetracycline

(c) Both of these          (d) None of these

C

25.   

Which of the following components is necessary to carry out a PCR reaction?

(a) Template DNA

(b) Deoxy Ribonucleoside, Triphosphates (dNTPs)

(c) Primers

(d) Taq Polymerase

(c) All of these

E

26.   

In PCR, the complete denaturation of DNA takes place at a temperature of:

(a) 54°C                       (b) 20°C

(c) 10°C                       (d) 94 C

D

27.   

A DNA sequencing method called chemical cleavage method was developed by:

(3) Maxam and Gilbert.

(b) Hershey and Chase

(c) Watson and Crick

(d) Beadle and Tatum

A

28.   

A collection of bacterial or bacteriophage clones, each containing at least one copy of

every DNA sequence in a genome of an organism is known as:

(a) DNA Probe

(b) DNA Fingerprinting

(c) Genomic Library

(d) PCR

C

29.   

Southern blotting method is used for the detection of a specified:

(a) Hormone                (b) Enzyme

(c) Protein                    (d) DNA Sequence

D

30.   

Which of the following is a commonly used DNA marker?

(a) RFLPS                   

(b) VNTRS

(c) SNP                                   

(d) Microsatellite Polymorphism

(e) All of these

E

31.   

Human genome project revealed that human DNA is made up of:

(a) 25000 Base Pairs

(b) 300 Base Pairs

(c) 3 Million Base Pairs

(d) 3 Billion Base Pairs

D

32.   

The initial plant part which is used to develop tissue culture is called:

(a) Callus

(b) Explant

(c) Cell-Suspension Culture

(d) Somatic Embryo

B

33.   

A culture technique which involves recombination of different cell types to form a more defined tissue or an organ is known as:

(a) Tissue Culture        (b) Organotypic Culture

(c) Anther Culture        (d) Protoplasts Culture

A

34.   

The first genes available for genetic engineering of crop plants for pest resistance

were:

(a) SRY Gene              (b) Polygenes

(c) Cry Genes              (d) None of these

C

35.   

First transgenic bacterium was produced in:

(a) 1906                       (b) 1941

(c) 1928.                      (d) 1973

D

36.   

Removal of environmental pollutants by organisms is known as:

(a) Bioremediation       (b) Biosafety

(c) Biosynthesis                       (d) Transformation

A

37.   

Somatic cell hybridization technique is used for the production of:

(a) Transgenic Animals            (b) Monoclonal Antibodies

(c) DNA Probes                       (d) Liposomes

B

38.   

A technique for correcting defective genes responsible for disease development is known as:

(a) Genetic Engineering

(b) Tissue Culture

(c) Recombinant DNA Technology

(d) Gene Therapy

D

39.   

Radioactive or fluorescent single stranded nucleotide sequence that will hybridize (pair) with a certain piece of DNA to search a genetic library for a certain gene is known as:

(a) Probe                      (b) Recombinant DNA

(c) Promoter                 (d Vector

A

40.   

First DNA fingerprint was made in 1985 by:

(a) Landsteiner             (b) A. Jeffrey

(c) Bateson                  (d) Sturtevant

B

41.   

Liposome, which carries the therapeutic DNA. is chemically:

(a) Carbohydrate          (b) Protein

(c) Hormone                (d) Lipid

D

42.   

The human genome is times larger than any other genome sequenced so far.

(a) 2                             (b) 5

© 25                            (d) 60

A

43.   

Which of these is a true statement?

(a) Both plasmids and viruses can serve as Vectors

(b) Plasmids can carry recombinant DNA but viruses cannot

© Vectors carry only the foreign gene into the host cell

(d) Only gene therapy uses vectors

(e) Both a and d are correct

E

44.   

Which of these is a benefit to having insulin. produced by biotechnology?

(a) It is just as effective

(b) It can be mass produced

(c) It is non-allergic

(d) It is less expensive

(e) All of these are correct

E

45.   

Restriction fragment length polymorphism (RFLPS):

(a) Are achieved by using restriction enzymes

(b) Identify individuals genetically

(c) Are the basis for DNA finger prints

(d) Can be subjected to gel electrophoresis

(e) All of these are correct

E

46.   

Which of these would you not expect to be a biotechnology product?

(a) Vaccine                  (b) Modified Enzymes

(C) Protein Hormones  (d) DNA Probes

(e) Steroid Hormones

E

47.   

What is the benefit of using a retrovirus as a vector in gene therapy?

(a) It is not able to enter the cell

(b) It incorporates the foreign gene into the host chromosome

(c) It prevents infection by other viruses

(d) It eliminates a lot of unnecessary steps

B

48.   

Gel electrophoresis:

(a) Cannot be used on nucleotide

(b) Measures the size of plasmids

(C) Tells whether viruses are infectious

(d) Measures the change and size of proteins and DNA fragments

(e) All of these are correct

D

49.   

Which of these is incorrectly matched?

(a) Protoplast-plant cell engineering

(b) RFLPS-DNA fingerprinting

(C) DNA polymerase – PCR

(d) DNA ligase-mapping human chromosomes.

D

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